Hotspot

clpP

LSC · 69463-71516 · 2054 bp

Back to Melanoseris

Pi 0.00127

MarkerSeek score 59.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2054
variable_sites: 6
pi: 0.00127
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGATGCGAAATCCAGGCT	GGCTTTGTTACGTTTCCACA	45.0	45.0	57.9	56.9	2130-2141 bp	1.0	71.2
clpP_p2	CTCGGCAGGGGTAATTTCAA	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2157-2168 bp	1.0	70.7
clpP_p3	AACTCGGCAGGGGTAATTTC	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2159-2170 bp	1.0	70.7
clpP_p4	GAAACTCGGCAGGGGTAATT	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2161-2172 bp	1.0	70.7
clpP_p5	TATGAAACTCGGCAGGGGTA	GGCTTTGTTACGTTTCCACA	50.0	45.0	58.1	56.9	2164-2175 bp	1.0	70.6