Hotspot

accD-psaI

LSC · 58118-58844 · 727 bp

Back to Melanoseris

Pi 0.0033
MarkerSeek score 50.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
727
variable_sites
6
pi
0.0033
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 ACACACTTTCTTTCCTTTGAATCA AAAGAAGCCATTGCGATTGC 33.3 45.0 57.2 58.0 823-835 bp 1.0 43.5
accD-psaI_p2 ACACACTTTCTTTCCTTTGAATCA TTGCGATTGCCGGAAATACT 33.3 45.0 57.2 58.3 813-825 bp 1.0 41.8
accD-psaI_p3 ACACACTTTCTTTCCTTTGAATCA GCCGGAAATACTAGGCCTAC 33.3 55.0 57.2 57.5 805-817 bp 1.0 41.8
accD-psaI_p4 ACACACTTTCTTTCCTTTGA AAAGAAGCCATTGCGATTGC 35.0 45.0 52.8 58.0 823-835 bp 1.0 41.7
accD-psaI_p5 ACACACTTTCTTTCCTTTGAATCAA AAAGAAGCCATTGCGATTGC 32.0 45.0 57.8 58.0 823-835 bp 1.0 41.7