Hotspot

ycf3

LSC · 44504-46502 · 1999 bp

Back to Melanosciadium

Pi 0.00334

MarkerSeek score 46.30

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1999
variable_sites: 10
pi: 0.00334
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ACAAAGAGACCTGCCAACAG	TCGGTTTACCACGAGAGGTA	50.0	50.0	58.0	57.8	2732-2754 bp	1.0	78.7
ycf3_p2	CAAAGAGACCTGCCAACAGT	TCGGTTTACCACGAGAGGTA	50.0	50.0	58.0	57.8	2731-2753 bp	1.0	78.7
ycf3_p3	ACCCAGACGAAATAGAACGC	TCGGTTTACCACGAGAGGTA	50.0	50.0	58.0	57.8	2238 bp	0.333333	51.7
ycf3_p4	ACCCAGACGAAATAGAACGC	GATCGGTTTACCACGAGAGG	50.0	55.0	58.0	57.8	2240 bp	0.333333	51.6
ycf3_p5	ACCCAGACGAAATAGAACGC	GGATCGGTTTACCACGAGAG	50.0	55.0	58.0	57.8	2241 bp	0.333333	51.6