Hotspot

petB

IRb · 76582-77984 · 1403 bp

Back to Melanosciadium

Pi 0.00618

MarkerSeek score 49.50

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: IRb
length_bp: 1403
variable_sites: 13
pi: 0.00618
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TCCTTTGATGGGTGTTGCAA	ATGCGGCAGATGGATAAAGG	45.0	50.0	57.9	58.1	1881 bp	0.333333	51.9
petB_p2	TCCTTTGATGGGTGTTGCAA	CGGCAGATGGATAAAGGCAT	45.0	50.0	57.9	58.1	1878 bp	0.333333	51.9
petB_p3	AGCTCCTTTGATGGGTGTTG	ATGCGGCAGATGGATAAAGG	50.0	50.0	58.1	58.1	1884 bp	0.333333	51.9
petB_p4	AGCTCCTTTGATGGGTGTTG	CGGCAGATGGATAAAGGCAT	50.0	50.0	58.1	58.1	1881 bp	0.333333	51.9
petB_p5	CAGCTCCTTTGATGGGTGTT	CGGCAGATGGATAAAGGCAT	50.0	50.0	58.1	58.1	1882 bp	0.333333	51.9