Hotspot

rpl20-rps12

LSC · 70501-71280 · 780 bp

Back to Melanosciadium

Pi 0.00343

MarkerSeek score 61.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 4
pi: 0.00343
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATTTCCCCGT	CAGCGTCGAGGAACATGTAC	50.0	55.0	58.0	58.7	842-844 bp	1.0	74.7
rpl20-rps12_p2	TCCGAGCTATATTTCCCCGT	AGCGTCGAGGAACATGTACT	50.0	50.0	58.0	58.8	841-843 bp	1.0	73.9
rpl20-rps12_p3	TCCGAGCTATATTTCCCCGT	TCAGCGTCGAGGAACATGTA	50.0	50.0	58.0	58.8	843-845 bp	1.0	73.9
rpl20-rps12_p4	TCCGAGCTATATTTCCCCGT	GCGTCGAGGAACATGTACTA	50.0	50.0	58.0	57.2	840-842 bp	1.0	73.8
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	896-898 bp	1.0	73.8