Hotspot

rbcL-accD

LSC · 58925-59563 · 639 bp

Back to Melanosciadium

Pi 0.0077

MarkerSeek score 61.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 7
pi: 0.0077
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTGCTTGTGAAGTATG	CACACCTGTGTTCGAACTCT	50.0	50.0	57.5	57.8	727-760 bp	1.0	78.2
rbcL-accD_p2	GCTGCTGCTTGTGAAGTATG	TAGACCACACCTGTGTTCGA	50.0	50.0	57.5	58.3	732-765 bp	1.0	77.6
rbcL-accD_p3	CGTGAGGCTGCTAAATGGAG	CACACCTGTGTTCGAACTCT	55.0	50.0	58.7	57.8	757-790 bp	1.0	76.7
rbcL-accD_p4	GCTGCTGCTTGTGAAGTATG	TTCAATAGGACCAAGACCGC	50.0	50.0	57.5	57.6	765-798 bp	1.0	76.6
rbcL-accD_p5	CGTGAGGCTGCTAAATGGAG	TAGACCACACCTGTGTTCGA	55.0	50.0	58.7	58.3	762-795 bp	1.0	76.0