Hotspot

petN-psbM

LSC · 30202-31349 · 1148 bp

Back to Melanosciadium

Pi 0.00467

MarkerSeek score 62.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 8
pi: 0.00467
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATCC	50.0	50.0	58.2	57.9	1316-1322 bp	1.0	78.9
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATCC	50.0	50.0	57.6	57.9	1255-1261 bp	1.0	77.4
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	GCTACTGCACTGTTCATCCT	50.0	50.0	58.2	57.6	1315-1321 bp	1.0	76.6
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GCTACTGCACTGTTCATCCT	50.0	50.0	57.6	57.6	1254-1260 bp	1.0	75.0
petN-psbM_p5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATCC	50.0	50.0	57.1	57.9	1317-1323 bp	1.0	73.0