Hotspot

atpF-atpH

LSC · 14132-14430 · 299 bp

Back to Melanosciadium

Pi 0.00904

MarkerSeek score 61.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 299
variable_sites: 4
pi: 0.00904
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	407-415 bp	1.0	79.4
atpF-atpH_p2	CCAGTGGCCTAAAGAAACGA	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.8	58.2	380-388 bp	1.0	79.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	409-417 bp	1.0	78.0
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGAGGCGGAGGGAAAAATAC	50.0	50.0	57.9	57.6	478-486 bp	1.0	77.7
atpF-atpH_p5	CCAGTGGCCTAAAGAAACGA	TGAGGCGGAGGGAAAAATAC	50.0	50.0	57.8	57.6	451-459 bp	1.0	77.4