Hotspot

trnS-GCU-trnG-UCC

LSC · 9560-10120 · 561 bp

Back to Melanosciadium

Pi 0.00713

MarkerSeek score 63.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 6
pi: 0.00713
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	GGGGGTTACTCGAACGAATC	45.0	55.0	58.0	58.1	648-661 bp	1.0	80.4
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	GGGGGTTACTCGAACGAATC	50.0	55.0	58.2	58.1	697-710 bp	1.0	79.4
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	TAAGGGGGTTACTCGAACGA	45.0	50.0	58.0	57.8	651-664 bp	1.0	79.2
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	TAAGGGGGTTACTCGAACGA	50.0	50.0	58.2	57.8	700-713 bp	1.0	78.2
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	AGGGGGTTACTCGAACGAAT	45.0	50.0	58.0	58.4	649-662 bp	1.0	77.4