Hotspot

psaC-rps15

SSC · 112378-113233 · 856 bp

Back to Melampyrum

Pi 0.0331

MarkerSeek score 72.90

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: SSC
length_bp: 856
variable_sites: 53
pi: 0.0331
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	GCTCGGACACATTGAGTACA	TGGGGCGTAATACCATTTGA	50.0	45.0	57.6	56.9	908-935 bp	1.0	76.6
psaC-rps15_p2	GCTCGGACACATTGAGTACA	TTGGGGCGTAATACCATTTGA	50.0	42.9	57.6	57.6	909-936 bp	1.0	74.5
psaC-rps15_p3	CGCTCGGACACATTGAGTAC	TGGGGCGTAATACCATTTGA	55.0	45.0	58.7	56.9	909-936 bp	1.0	73.7
psaC-rps15_p4	CGCTCGGACACATTGAGTAC	TTGGGGCGTAATACCATTTGA	55.0	42.9	58.7	57.6	910-937 bp	1.0	71.5
psaC-rps15_p5	GCTCGGACACATTGAGTACAT	TGGGGCGTAATACCATTTGA	47.6	45.0	58.1	56.9	908-935 bp	1.0	70.7