Hotspot

ndhD-psaC

SSC · 110916-112131 · 1216 bp

Back to Melampyrum

Pi 0.032

MarkerSeek score 70.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 1216
variable_sites: 76
pi: 0.032
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CCATAGGAACCCTTTCAGGC	GGGGCGGATGTAAAGCTAAA	55.0	50.0	57.9	58.0	2032-2092 bp	1.0	87.2
ndhD-psaC_p2	CCTTGTCAGCTGATCGTGAA	CAAATTGCTTCTGCTCCACG	50.0	50.0	57.9	58.0	1592-1621 bp	0.4	57.8
ndhD-psaC_p3	TCAACCTTGTCAGCTGATCG	CAAATTGCTTCTGCTCCACG	50.0	50.0	57.9	58.0	1596-1625 bp	0.4	57.8
ndhD-psaC_p4	CCATAGGAACCCTTTCAGGC	CAAATTGCTTCTGCTCCACG	55.0	50.0	57.9	58.0	2043-2072 bp	0.4	57.5
ndhD-psaC_p5	TTGGAGTTGGGACCCAAAAG	CAAATTGCTTCTGCTCCACG	50.0	50.0	57.9	58.0	1991-2020 bp	0.4	57.3