Hotspot

trnL-UAG-ccsA

SSC · 109382-109491 · 110 bp

Back to Melampyrum

Pi 0.0691

MarkerSeek score 77.00

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 110
variable_sites: 14
pi: 0.0691
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGGAAAATGGCCCGAAGAAA	50.0	45.0	57.9	58.0	372-374 bp	0.8	75.5
trnL-UAG-ccsA_p2	TGAATTCAAGACAAGCCGCT	TGGAAAATGGCCCGAAGAAA	45.0	45.0	58.1	58.0	405-407 bp	0.8	75.3
trnL-UAG-ccsA_p3	ACAAGCCGCTATGGTGAAAT	TGGAAAATGGCCCGAAGAAA	45.0	45.0	57.9	58.0	395-397 bp	0.8	75.2
trnL-UAG-ccsA_p4	AAGCATCTCGGTTCGAATCC	TGGAAAATGGCCCGAAGAAA	50.0	45.0	58.1	58.0	342 bp	0.2	47.5
trnL-UAG-ccsA_p5	AAGCATCTCGGTTCGAATCC	GAAGTAGCAAAGCCCTGTGT	50.0	50.0	58.1	58.1	485 bp	0.2	46.7