Hotspot

rpl22-rps19

LSC · 82805-82873 · 69 bp

Back to Melampyrum

Pi 0.0357

MarkerSeek score 66.00

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 5
pi: 0.0357
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ACGGGCTTGAGGTTTGAATT	CATGGGGTTATCCTGCACTC	45.0	55.0	58.0	58.0	779-795 bp	1.0	83.5
rpl22-rps19_p2	TTGCATAACTACGTCCACGG	CATGGGGTTATCCTGCACTC	50.0	55.0	58.0	58.0	797 bp	0.4	56.1
rpl22-rps19_p3	TTGCATAACTACGTCCACGG	TTGGGTAGAGCTGGATCCAA	50.0	50.0	58.0	58.0	930 bp	0.4	56.0
rpl22-rps19_p4	TTGCATAACTACGTCCACGG	TTTGGGTAGAGCTGGATCCA	50.0	50.0	58.0	58.0	931 bp	0.4	56.0
rpl22-rps19_p5	TTGCATAACTACGTCCACGG	GCGAATCAGAAACGTTTGGG	50.0	50.0	58.0	58.0	945 bp	0.4	56.0