Hotspot

trnF-GAA-ndhJ

LSC · 47620-48210 · 591 bp

Back to Melampyrum

Pi 0.0255

MarkerSeek score 69.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 591
variable_sites: 29
pi: 0.0255
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GCCCCCAATTTTTATGAAAAACA	55.0	34.8	58.0	56.7	697-714 bp	0.8	40.9
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	TGCCCCCAATTTTTATGAAAAAC	55.0	34.8	58.0	56.7	698-715 bp	0.8	40.9
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TGCCCCCAATTTTTATGAAAAACA	55.0	33.3	58.0	58.3	698-715 bp	0.8	40.8
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GCCCCCAATTTTTATGAAAAACA	50.0	34.8	58.1	56.7	661-678 bp	0.8	40.7
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGCCCCCAATTTTTATGAAAAAC	50.0	34.8	58.1	56.7	662-679 bp	0.8	40.7