Hotspot

trnT-UGU-trnL-UAA

LSC · 45987-46718 · 732 bp

Back to Melampyrum

Pi 0.0295

MarkerSeek score 70.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 43
pi: 0.0295
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	794-871 bp	1.0	81.5
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	826-903 bp	1.0	80.5
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	788-865 bp	1.0	80.2
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	820-897 bp	1.0	79.2
trnT-UGU-trnL-UAA_p5	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.5	58.2	786-863 bp	1.0	78.6