Hotspot

trnE-UUC-trnT-GGU

LSC · 30312-30896 · 585 bp

Back to Melampyrum

Pi 0.0139
MarkerSeek score 65.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
585
variable_sites
15
pi
0.0139
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CTCCGATCACCTTCCTTTCG GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 733-747 bp 1.0 83.4
trnE-UUC-trnT-GGU_p2 GATCACCTTCCTTTCGCAGT GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 729-743 bp 1.0 83.2
trnE-UUC-trnT-GGU_p3 CTCCGATCACCTTCCTTTCG CCGATGACTTACGCCTTACC 55.0 55.0 58.1 58.2 730-744 bp 1.0 82.2
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 680-694 bp 1.0 82.2
trnE-UUC-trnT-GGU_p5 GATCACCTTCCTTTCGCAGT CCGATGACTTACGCCTTACC 50.0 55.0 57.9 58.2 726-740 bp 1.0 82.0