Hotspot

petN-psbM

LSC · 28313-29265 · 953 bp

Back to Melampyrum

Pi 0.0172

MarkerSeek score 67.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 35
pi: 0.0172
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TGCTACTGCACTATTCATTCT	50.0	38.1	58.0	54.7	1059-1094 bp	1.0	49.7
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TGCTACTGCACTATTCATTCT	50.0	38.1	58.0	54.7	1057-1092 bp	1.0	49.7
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TGCTACTGCACTATTCATTCT	55.0	38.1	57.9	54.7	1056-1091 bp	1.0	49.1
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTATTCATTCT	50.0	38.1	58.2	54.7	1111-1146 bp	1.0	48.3
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	TGCTACTGCACTATTCATTCTAGT	50.0	37.5	58.0	57.5	1057-1092 bp	1.0	48.0