Hotspot

rps15-ycf1

SSC · 128715-129154 · 440 bp

Back to Melaleuca

Pi 0.0439

MarkerSeek score 73.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 440
variable_sites: 26
pi: 0.0439
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACGGACCCCTTGTTTTCTTC	ATGGTAGCCGCTTCAGTATG	50.0	50.0	58.0	57.5	536-577 bp	1.0	83.6
rps15-ycf1_p2	ACGGACCCCTTGTTTTCTTC	TCCACTAAAGGGCGTAGAGA	50.0	50.0	58.0	57.5	768-809 bp	1.0	82.4
rps15-ycf1_p3	GATGAGCCGAAGAACACCAT	ATGGTAGCCGCTTCAGTATG	50.0	50.0	58.0	57.5	937-978 bp	1.0	80.6
rps15-ycf1_p4	GATGAGCCGAAGAACACCAT	TCCACTAAAGGGCGTAGAGA	50.0	50.0	58.0	57.5	1169-1210 bp	1.0	80.4
rps15-ycf1_p5	TCTGTGCACCAATATCTGCC	TCCACTAAAGGGCGTAGAGA	50.0	50.0	58.0	57.5	1467-1508 bp	1.0	79.5