Hotspot

rpl32-trnL-UAG

SSC · 118269-119273 · 1005 bp

Back to Melaleuca

Pi 0.0423
MarkerSeek score 67.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1005
variable_sites
40
pi
0.0423
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.64
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAATTAAATAGTCAAACA AGCGTGTCTACCGATTTCAC 32.0 50.0 58.0 58.0 728-1068 bp 1.0 47.7
rpl32-trnL-UAG_p2 TGTGCGACAATTAAATAGTCAAACA CAGCGTGTCTACCGATTTCA 32.0 50.0 58.0 58.0 729-1069 bp 1.0 47.7
rpl32-trnL-UAG_p3 TGTGCGACAATTAAATAGTCAAACA TCCTAAGAGCAGCGTGTCTA 32.0 50.0 58.0 57.9 738-1078 bp 1.0 47.7
rpl32-trnL-UAG_p4 TGCGACAATTAAATAGTCAAACACT AGCGTGTCTACCGATTTCAC 32.0 50.0 57.8 58.0 726-1066 bp 1.0 47.7
rpl32-trnL-UAG_p5 TGCGACAATTAAATAGTCAAACACT CAGCGTGTCTACCGATTTCA 32.0 50.0 57.8 58.0 727-1067 bp 1.0 47.7