Hotspot

rrn5-trnR-ACG

IRb · 112144-112389 · 246 bp

Back to Melaleuca

Pi 0.0328

MarkerSeek score 76.90

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: IRb
length_bp: 246
variable_sites: 12
pi: 0.0328
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	GGTGACGATACTGTAGGGGA	CCACGTGCTCTAATCCTCTG	55.0	55.0	57.9	57.8	319-330 bp	1.0	83.4
rrn5-trnR-ACG_p2	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	354-365 bp	1.0	82.3
rrn5-trnR-ACG_p3	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	357-368 bp	1.0	82.3
rrn5-trnR-ACG_p4	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	355-366 bp	1.0	82.3
rrn5-trnR-ACG_p5	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	358-369 bp	1.0	82.3