Hotspot

petA-psbJ

LSC · 66686-67686 · 1001 bp

Back to Melaleuca

Pi 0.04

MarkerSeek score 76.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1001
variable_sites: 57
pi: 0.04
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATACTCCGGGTTGGGTTCAT	50.0	50.0	57.8	58.4	1108-1146 bp	1.0	81.8
petA-psbJ_p2	GATCCATTCCGTGTCCAAGG	ATACTCCGGGTTGGGTTCAT	55.0	50.0	58.3	58.4	1126-1164 bp	1.0	80.3
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	TTCATACTCCGGGTTGGGTT	50.0	50.0	57.8	58.6	1111-1149 bp	1.0	80.0
petA-psbJ_p4	TGTCCAAGGCCTTTTGTTCT	ATACTCCGGGTTGGGTTCAT	45.0	50.0	57.5	58.4	1115-1153 bp	1.0	79.4
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	ATGGTTCATACTCCGGGTTG	50.0	50.0	57.8	57.3	1115-1153 bp	1.0	79.4