Hotspot

psbZ-trnG-GCC

LSC · 38864-39728 · 865 bp

Back to Melaleuca

Pi 0.0841
MarkerSeek score 84.60
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
865
variable_sites
80
pi
0.0841
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.91
markerseek_score
84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 AGTCTTTCTGGTGGGTATCCT CGTCTTCTCCTTGGCAAAGA 47.6 50.0 57.8 57.8 752-973 bp 1.0 80.2
psbZ-trnG-GCC_p2 AGTCTTTCTGGTGGGTATCC CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.2 57.8 752-973 bp 1.0 75.6
psbZ-trnG-GCC_p3 GTCTTTCTGGTGGGTATCCT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.2 57.8 751-972 bp 1.0 75.6
psbZ-trnG-GCC_p4 TGGATTAGTCTTTCTGGTGGG CGTCTTCTCCTTGGCAAAGA 47.6 50.0 57.0 57.8 758-979 bp 1.0 73.8
psbZ-trnG-GCC_p5 GTCTTTCTGGTGGGTATCCTT CGTCTTCTCCTTGGCAAAGA 47.6 50.0 57.0 57.8 751-972 bp 1.0 73.8