Hotspot

atpH-atpI

LSC · 14652-15753 · 1102 bp

Back to Melaleuca

Pi 0.0224

MarkerSeek score 70.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 35
pi: 0.0224
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1113-1159 bp	1.0	84.1
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	1169-1215 bp	1.0	83.2
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	1151-1197 bp	1.0	83.2
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1114-1160 bp	1.0	82.9
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.2	57.8	1173-1219 bp	1.0	82.6