Hotspot

trnR-UCU-atpA

LSC · 10649-10990 · 342 bp

Back to Melaleuca

Pi 0.0374

MarkerSeek score 69.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 15
pi: 0.0374
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.1	353-439 bp	1.0	72.8
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	CAGGAACAGAAGGAACGCTT	47.6	50.0	57.3	58.1	320-406 bp	1.0	72.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TTCACTGAGGAAGCAGAAGC	47.6	50.0	57.3	57.8	359-445 bp	1.0	72.0
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	AGGAAGCAGAAGCCCTTTTG	47.6	50.0	57.3	58.4	352-438 bp	1.0	70.5
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	351-437 bp	1.0	70.4