Hotspot

trnS-GCU-trnG-UCC

LSC · 8881-9611 · 731 bp

Back to Melaleuca

Pi 0.0276

MarkerSeek score 70.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 30
pi: 0.0276
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AAATCAAACCGAGGGATCCC	50.0	50.0	58.0	57.6	841-902 bp	1.0	80.5
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AAATCAAACCGAGGGATCCC	50.0	50.0	58.0	57.6	840-901 bp	1.0	80.5
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AAATCAAACCGAGGGATCCC	50.0	50.0	58.2	57.6	895-956 bp	1.0	79.1
trnS-GCU-trnG-UCC_p4	TGGAAACGGAAAGAGAGGGA	AAATCAAACCGAGGGATCCC	50.0	50.0	58.3	57.6	900-961 bp	1.0	78.1
trnS-GCU-trnG-UCC_p5	AGCAATCCGACGCTTTAGTC	AAATCAAACCGAGGGATCCC	50.0	50.0	58.4	57.6	843-904 bp	1.0	77.7