Hotspot

rps16-trnQ-UUG

LSC · 6084-7515 · 1432 bp

Back to Melaleuca

Pi 0.0298

MarkerSeek score 71.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1432
variable_sites: 62
pi: 0.0298
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1480-1505 bp	1.0	85.6
rps16-trnQ-UUG_p2	GGGTGGGTGTAAGAATCCAC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	57.9	58.1	1517-1542 bp	1.0	85.5
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1491-1516 bp	1.0	85.0
rps16-trnQ-UUG_p4	GGGTGGGTGTAAGAATCCAC	TTCGGAGGTTCGAATCCTTC	55.0	50.0	57.9	57.3	1521-1546 bp	1.0	80.8
rps16-trnQ-UUG_p5	GGGTGGGTGTAAGAATCCAC	TAAGGCAACGGGTTTTGGTC	55.0	50.0	57.9	58.7	1547-1572 bp	1.0	80.5