Hotspot

trnH-GUG-psbA

LSC · 81-509 · 429 bp

Back to Melaleuca

Pi 0.0452
MarkerSeek score 74.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
429
variable_sites
27
pi
0.0452
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 498-560 bp 1.0 83.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 512-574 bp 1.0 83.6
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 511-573 bp 1.0 83.6
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 513-575 bp 1.0 83.5
trnH-GUG-psbA_p5 CGCATGGTGGATTCACAATC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.5 57.9 527-589 bp 1.0 80.6