Hotspot

rpl32-trnL

SSC · 113214-114120 · 907 bp

Back to Megacarpaea

Pi 0.0304

MarkerSeek score 75.00

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 907
variable_sites: 60
pi: 0.0304
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	TCACTTTCTACAGGTAATTCAA	AGCAGCGTGTCTACCAATTT	31.8	45.0	52.8	57.8	937-1032 bp	1.0	48.9
rpl32-trnL_p2	TCACTTTCTACAGGTAATTCAA	TCCTAAGAGCAGCGTGTCTA	31.8	50.0	52.8	57.9	944-1039 bp	1.0	48.8
rpl32-trnL_p3	TCACTTTCTACAGGTAATTCAA	CCTAAGAGCAGCGTGTCTAC	31.8	55.0	52.8	57.8	943-1038 bp	1.0	48.8
rpl32-trnL_p4	TCACTTTCTACAGGTAATTCAA	CTAAGAGCAGCGTGTCTACC	31.8	55.0	52.8	57.8	942-1037 bp	1.0	48.8
rpl32-trnL_p5	TCACTTTCTACAGGTAATTCAA	TCGAACCGAGATGCTTTAGC	31.8	50.0	52.8	58.1	970-1065 bp	1.0	48.7