Hotspot

ndhF-rpl32

SSC · 112245-113054 · 810 bp

Back to Megacarpaea

Pi 0.0248

MarkerSeek score 66.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 810
variable_sites: 43
pi: 0.0248
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCAATAGGTCGTGTGAACCA	AGGATGTCCAATATCCCTTCCT	50.0	45.5	58.2	58.0	1378-1407 bp	1.0	67.3
ndhF-rpl32_p2	TGCAATAGGTCGTGTGAACC	AGGATGTCCAATATCCCTTCCT	50.0	45.5	58.2	58.0	1379-1408 bp	1.0	67.3
ndhF-rpl32_p3	GGTCGTGTGAACCAAAAACC	AGGATGTCCAATATCCCTTCCT	50.0	45.5	57.8	58.0	1372-1401 bp	1.0	67.3
ndhF-rpl32_p4	GGAAGCGGAATGAAAGGGAT	AGGATGTCCAATATCCCTTCCT	50.0	45.5	57.9	58.0	930-945 bp	0.8	62.0
ndhF-rpl32_p5	ACTGGAAGCGGAATGAAAGG	AGGATGTCCAATATCCCTTCCT	50.0	45.5	58.2	58.0	933-948 bp	0.8	61.0