Hotspot

rpl36-rps8

LSC · 79050-79522 · 473 bp

Back to Megacarpaea

Pi 0.0352

MarkerSeek score 68.10

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 39
pi: 0.0352
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CCTTGGAAGTGCCTTTTCCT	GAACGGTTCGGATAGGATCG	50.0	55.0	58.0	58.0	966-979 bp	1.0	84.6
rpl36-rps8_p2	TCCTTGGAAGTGCCTTTTCC	GAACGGTTCGGATAGGATCG	50.0	55.0	58.0	58.0	967-980 bp	1.0	84.6
rpl36-rps8_p3	GCCCTCTCCTACGGATTAGT	GAACGGTTCGGATAGGATCG	55.0	55.0	58.0	58.0	878-898 bp	1.0	84.3
rpl36-rps8_p4	AGTCCAGGGCCTTTTATCCT	GAACGGTTCGGATAGGATCG	50.0	55.0	58.0	58.0	1335-1350 bp	1.0	83.6
rpl36-rps8_p5	CTTCAGCTCGTTGCATACCT	GAACGGTTCGGATAGGATCG	50.0	55.0	58.0	58.0	1313-1328 bp	1.0	83.6