Hotspot

petD-rpoA

LSC · 77133-77351 · 219 bp

Back to Megacarpaea

Pi 0.038

MarkerSeek score 67.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 219
variable_sites: 16
pi: 0.038
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CCGTCTTTTTGATTGGCACC	GAAGCCTCCCGGAATTTGAT	50.0	50.0	57.9	57.9	627-653 bp	1.0	85.2
petD-rpoA_p2	CCGTCTTTTTGATTGGCACC	CGCGCTTATTTGTGTCCAAG	50.0	50.0	57.9	58.1	961-987 bp	1.0	83.2
petD-rpoA_p3	TGGTACCATTGCCTGTAACG	GAAGCCTCCCGGAATTTGAT	50.0	50.0	57.9	57.9	884-910 bp	1.0	83.1
petD-rpoA_p4	GGTACCATTGCCTGTAACGT	GAAGCCTCCCGGAATTTGAT	50.0	50.0	57.9	57.9	883-909 bp	1.0	83.1
petD-rpoA_p5	AAGCTTACGTGGGTGTTGAG	GAAGCCTCCCGGAATTTGAT	50.0	50.0	58.1	57.9	1247-1273 bp	1.0	82.2