Hotspot

psbE-ORF31

LSC · 64155-65109 · 955 bp

Back to Megacarpaea

Pi 0.0235

MarkerSeek score 69.30

Primer available yes

Label psbE-ORF31

Feature Metadata

label_name: psbE-ORF31
feature_type: igs
region: LSC
length_bp: 955
variable_sites: 48
pi: 0.0235
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-ORF31_p1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.3	1106-1554 bp	1.0	70.5
psbE-ORF31_p2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1024-1472 bp	1.0	70.1
psbE-ORF31_p3	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1107-1555 bp	1.0	69.8
psbE-ORF31_p4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1019-1467 bp	1.0	69.8
psbE-ORF31_p5	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGAA	45.0	42.9	57.9	57.0	1106-1554 bp	1.0	68.6