Hotspot

rbcL-accD

LSC · 56077-56762 · 686 bp

Back to Megacarpaea

Pi 0.0215

MarkerSeek score 66.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 28
pi: 0.0215
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	TTTGAACCACGATTTTTCCA	55.0	35.0	57.7	53.9	652-802 bp	1.0	51.9
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	TCAATTTGAACCACGATTTTTCCA	55.0	33.3	57.7	58.1	656-806 bp	1.0	51.8
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	AGAAAACATCAATTTGAACCACGA	55.0	33.3	57.7	57.9	664-814 bp	1.0	51.4
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	CAATTTGAACCACGATTTTTCCA	55.0	34.8	57.7	56.8	655-805 bp	1.0	50.7
rbcL-accD_p5	GCTTGCAAATGGAGTCCTGA	TTTGAACCACGATTTTTCCA	50.0	35.0	58.5	53.9	663-813 bp	1.0	50.5