Hotspot

trnT-psbD

LSC · 31389-32647 · 1259 bp

Back to Megacarpaea

Pi 0.0174

MarkerSeek score 66.40

Primer available yes

Label trnT-psbD

Feature Metadata

label_name: trnT-psbD
feature_type: igs
region: LSC
length_bp: 1259
variable_sites: 48
pi: 0.0174
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1336-1414 bp	1.0	85.9
trnT-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1339-1417 bp	1.0	85.7
trnT-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.3	58.0	1313-1391 bp	1.0	84.2
trnT-psbD_p4	GGCCCTTTTAACTCAGTGGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	57.7	58.0	1351-1429 bp	1.0	84.1
trnT-psbD_p5	TGGTAGAGTAACGCCATGGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.4	58.0	1335-1413 bp	1.0	83.0