Hotspot

trnD-trnY

LSC · 29866-30300 · 435 bp

Back to Megacarpaea

Pi 0.0324

MarkerSeek score 64.10

Primer available yes

Label trnD-trnY

Feature Metadata

label_name: trnD-trnY
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 32
pi: 0.0324
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-trnY_p1	ATTTTTACCCCCTTGCCCAG	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.0	57.9	795-799 bp	1.0	83.1
trnD-trnY_p2	AAAGCAGAAACCCCGTACAG	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.1	57.9	969-971 bp	0.8	74.2
trnD-trnY_p3	GACAAAAAGCAGAAACCCCG	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.9	57.9	974-976 bp	0.8	73.9
trnD-trnY_p4	GGCGGTGCTCTAACCAATTA	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.0	57.9	691 bp	0.2	47.0
trnD-trnY_p5	GGCGGTGCTCTAACCAATTA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	510 bp	0.2	45.8