Hotspot

trnC-ycf6

LSC · 27377-27996 · 620 bp

Back to Megacarpaea

Pi 0.0162

MarkerSeek score 57.50

Primer available yes

Label trnC-ycf6

Feature Metadata

label_name: trnC-ycf6
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 25
pi: 0.0162
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-ycf6_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	729-737 bp	1.0	82.4
trnC-ycf6_p2	GTTTCTATCGTTTTGGCGGC	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	785-793 bp	1.0	81.9
trnC-ycf6_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	730-738 bp	1.0	81.8
trnC-ycf6_p4	TTTCCCCAGTTCAAATCCGG	CCCCATGAATGGTTAACGGT	50.0	50.0	58.1	57.9	917-926 bp	1.0	81.5
trnC-ycf6_p5	GTTTCTATCGTTTTGGCGGC	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	786-794 bp	1.0	81.3