Hotspot

trnS-trnT

LSC · 7834-8443 · 610 bp

Back to Megacarpaea

Pi 0.0286

MarkerSeek score 68.10

Primer available yes

Label trnS-trnT

Feature Metadata

label_name: trnS-trnT
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 38
pi: 0.0286
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnT_p1	CCAACGCTTTAGTCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	57.8	55.5	675-763 bp	1.0	55.1
trnS-trnT_p2	AACGCTTTAGTCCACTCAGC	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	673-761 bp	1.0	54.8
trnS-trnT_p3	CCAACGCTTTAGTCCACTCA	GAACGAATCACACTTTTACCACT	50.0	39.1	57.8	57.3	677-765 bp	1.0	54.1
trnS-trnT_p4	AACGCTTTAGTCCACTCAGC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	675-763 bp	1.0	53.8
trnS-trnT_p5	CCAACGCTTTAGTCCACTCA	AACGAATCACACTTTTACCACT	50.0	36.4	57.8	56.2	676-764 bp	1.0	53.2