Hotspot

psbK-psbI

LSC · 7246-7538 · 293 bp

Back to Megacarpaea

Pi 0.0349

MarkerSeek score 70.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 293
variable_sites: 22
pi: 0.0349
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	TGGCAAGCAGCTGTAAGTTT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.3	54.4	338-346 bp	1.0	47.0
psbK-psbI_p2	TTGGCAAGCAGCTGTAAGTT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.3	54.4	339-347 bp	1.0	47.0
psbK-psbI_p3	TTTGGCAAGCAGCTGTAAGT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.3	54.4	340-348 bp	1.0	47.0
psbK-psbI_p4	GTTTGGCAAGCAGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCA	50.0	31.8	57.9	54.4	341-349 bp	1.0	46.9
psbK-psbI_p5	GTTTGGCAAGCAGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCAT	50.0	30.4	57.9	55.0	341-349 bp	1.0	46.9