Hotspot

rps16

LSC · 4911-5928 · 1018 bp

Back to Meeboldia

Pi 0.00152

MarkerSeek score 62.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1018
variable_sites: 3
pi: 0.00152
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACCCCTTTCTATTTCTTTATTTCCT	TGTATCGACGGAACCAATGAC	32.0	47.6	56.2	58.1	1122-1155 bp	1.0	42.7
rps16_p2	ACCCCTTTCTATTTCTTTATTTCCT	GTATCGACGGAACCAATGACT	32.0	47.6	56.2	57.8	1121-1154 bp	1.0	42.7
rps16_p3	ACCCCTTTCTATTTCTTTATTTCCT	TGTATCGACGGAACCAATGA	32.0	45.0	56.2	56.7	1122-1155 bp	1.0	42.7
rps16_p4	ACCCCTTTCTATTTCTTTATTTCC	TGTATCGACGGAACCAATGAC	33.3	47.6	54.8	58.1	1122-1155 bp	1.0	42.7
rps16_p5	ACCCCTTTCTATTTCTTTATTTCCTT	TGTATCGACGGAACCAATGAC	30.8	47.6	56.8	58.1	1122-1155 bp	1.0	42.7