Hotspot

ndhH

SSC · 123661-124842 · 1182 bp

Back to Meeboldia

Pi 0.00268

MarkerSeek score 45.80

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 6
pi: 0.00268
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	ACACCAATACTGACATCGGC	AACGAAGACAACAAGGGGTC	50.0	50.0	58.0	58.0	1897-1904 bp	1.0	80.1
ndhH_p2	CCTGACATAAGAAGGCCCAC	AACGAAGACAACAAGGGGTC	55.0	50.0	58.0	58.0	1951-1958 bp	1.0	80.0
ndhH_p3	ACACCAATACTGACATCGGC	ACCTCTTTTCGCTTTCAGCA	50.0	45.0	58.0	58.0	1626 bp	1.0	79.9
ndhH_p4	CCTGACATAAGAAGGCCCAC	ACCTCTTTTCGCTTTCAGCA	55.0	45.0	58.0	58.0	1680 bp	1.0	79.8
ndhH_p5	ATACGTTGTTGTATCCCCGC	ACCTCTTTTCGCTTTCAGCA	50.0	45.0	58.1	58.0	1419 bp	1.0	79.7