Hotspot

rpl32-trnL-UAG

SSC · 115068-115919 · 852 bp

Back to Meeboldia

Pi 0.0092
MarkerSeek score 65.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
852
variable_sites
15
pi
0.0092
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTAAGGGATATTGGGCAGC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 983-995 bp 1.0 80.5
rpl32-trnL-UAG_p2 GTAAGGGATATTGGGCAGCC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 982-994 bp 1.0 80.5
rpl32-trnL-UAG_p3 GGTAAGGGATATTGGGCAGC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 984-996 bp 1.0 80.5
rpl32-trnL-UAG_p4 GTAAGGGATATTGGGCAGCC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 983-995 bp 1.0 80.5
rpl32-trnL-UAG_p5 GTAAGGGATATTGGGCAGCC CTCGCACTGCTTCCTAAGAG 55.0 55.0 58.1 58.1 1003-1015 bp 1.0 79.9