Hotspot

rps11-rpl36

LSC · 80628-80745 · 118 bp

Back to Meeboldia

Pi 0.0339

MarkerSeek score 63.00

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 118
variable_sites: 8
pi: 0.0339
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TCTCGGTATAGTTTTTGCCA	TTTGTTCCAACCCAAGACAT	40.0	40.0	54.4	55.3	177-182 bp	1.0	43.6
rps11-rpl36_p2	TCTCGGTATAGTTTTTGCCA	ATTTGTTCCAACCCAAGACA	40.0	40.0	54.4	55.3	178-183 bp	1.0	43.5
rps11-rpl36_p3	TTCTCGGTATAGTTTTTGCCA	ATTTGTTCCAACCCAAGACA	38.1	40.0	55.2	55.3	179-184 bp	1.0	43.5
rps11-rpl36_p4	TTCTCGGTATAGTTTTTGCCA	TTTGTTCCAACCCAAGACAT	38.1	40.0	55.2	55.3	178-183 bp	1.0	43.5
rps11-rpl36_p5	TCTCGGTATAGTTTTTGCCA	AATTTGTTCCAACCCAAGACA	40.0	38.1	54.4	56.2	179-184 bp	1.0	43.5