Hotspot

psaJ-rpl33

LSC · 68339-68812 · 474 bp

Back to Meeboldia

Pi 0.0097

MarkerSeek score 64.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 9
pi: 0.0097
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	TTTCGAACACAACCGGTACA	45.0	45.0	58.0	57.7	560-571 bp	1.0	80.2
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	TTTCGAACACAACCGGTACA	45.0	45.0	58.1	57.7	551-562 bp	1.0	79.8
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	TTTCGAACACAACCGGTACA	50.0	45.0	58.0	57.7	639-650 bp	1.0	79.7
psaJ-rpl33_p4	TTCCCAGATGCGTTGACATT	TTTCGAACACAACCGGTACA	45.0	45.0	57.8	57.7	558-569 bp	1.0	78.8
psaJ-rpl33_p5	TTTCCCAGATGCGTTGACAT	TTTCGAACACAACCGGTACA	45.0	45.0	57.8	57.7	559-570 bp	1.0	78.8