Hotspot

petA-psbJ

LSC · 64203-65174 · 972 bp

Back to Meeboldia

Pi 0.00365

MarkerSeek score 63.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 972
variable_sites: 6
pi: 0.00365
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.1	58.1	1141-1162 bp	1.0	72.3
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	1128-1149 bp	1.0	71.9
petA-psbJ_p3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.1	57.8	1142-1163 bp	1.0	71.5
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	1129-1150 bp	1.0	71.2
petA-psbJ_p5	CTTACGTGTCCAAGGCCTTT	TGGTTCGTATTCTGGATTGGG	50.0	47.6	58.1	57.7	1100-1121 bp	1.0	70.9