Hotspot

trnF-GAA-ndhJ

LSC · 49720-50105 · 386 bp

Back to Meeboldia

Pi 0.0117

MarkerSeek score 62.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 9
pi: 0.0117
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GAAAGTTGGGTAGGATGGCC	55.0	55.0	58.0	58.2	531 bp	1.0	79.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGGTAGGATGGCC	50.0	55.0	58.1	58.2	495 bp	1.0	79.2
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	GAAAGTTGGGTAGGATGGCC	55.0	55.0	57.9	58.2	519 bp	1.0	78.4
trnF-GAA-ndhJ_p4	AAATGAGGATGCGGTATCGG	GAAAGTTGGGTAGGATGGCC	50.0	55.0	57.8	58.2	565 bp	1.0	78.0
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGGTAGGATGGC	55.0	50.0	58.0	57.5	532 bp	1.0	77.3