Hotspot

petN-psbM

LSC · 29419-30548 · 1130 bp

Back to Meeboldia

Pi 0.00444

MarkerSeek score 62.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 10
pi: 0.00444
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1299-1308 bp	1.0	68.4
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1238-1247 bp	1.0	66.9
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	58.2	57.2	1278-1287 bp	1.0	65.5
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1299-1308 bp	1.0	64.3
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	57.6	57.2	1217-1226 bp	1.0	63.9