Hotspot

ycf1

SSC · 125111-130531 · 5421 bp

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Pi 0.014
MarkerSeek score 54.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5421
variable_sites
163
pi
0.014
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCATTGGAGAAGGAATTCGA CGGATTCAATGGTATCGATTAGT 40.0 39.1 54.1 56.3 167 bp 1.0 45.3
ycf1_p2 TCATTGGAGAAGGAATTCGA CGGATTCAATGGTATCGATT 40.0 40.0 54.1 53.3 167 bp 1.0 45.3
ycf1_p3 TTCATTGGAGAAGGAATTCGA CGGATTCAATGGTATCGATTAGT 38.1 39.1 54.9 56.3 168 bp 1.0 45.2
ycf1_p4 TTCATTGGAGAAGGAATTCGA CGGATTCAATGGTATCGATT 38.1 40.0 54.9 53.3 168 bp 1.0 45.2
ycf1_p5 TTTCATTGGAGAAGGAATTCGA CGGATTCAATGGTATCGATTAGT 36.4 39.1 55.7 56.3 169 bp 1.0 45.2