Hotspot

clpP

LSC · 70938-73103 · 2166 bp

Back to Medinilla

Pi 0.0121

MarkerSeek score 54.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2166
variable_sites: 54
pi: 0.0121
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TAGCTGACCCTCTTAGTCCG	AAACGCATTGTTACGCTTCC	55.0	45.0	57.9	57.7	2842-2896 bp	1.0	82.3
clpP_p2	AGTTGGCATGTTGAATCGGA	CGGAGGGATCAAAAACTGCT	45.0	50.0	57.8	58.2	2885-2940 bp	1.0	81.5
clpP_p3	TGACATTTCGGATTGGCTGT	CGGAGGGATCAAAAACTGCT	45.0	50.0	57.8	58.2	2931-2986 bp	1.0	81.5
clpP_p4	AGTTGGCATGTTGAATCGGA	TCGGAGGGATCAAAAACTGC	45.0	50.0	57.8	58.2	2886-2941 bp	1.0	81.5
clpP_p5	TGACATTTCGGATTGGCTGT	TCGGAGGGATCAAAAACTGC	45.0	50.0	57.8	58.2	2932-2987 bp	1.0	81.4