Hotspot

ndhB-trnL-CAA

IRa · 145039-145667 · 629 bp

Back to Medinilla

Pi 0.0103

MarkerSeek score 70.00

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 629
variable_sites: 13
pi: 0.0103
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	CCAGGAATATCAATGAACCCGA	GTCTCGCGTGTCTACCATTT	45.5	50.0	57.9	58.0	664-715 bp	1.0	68.8
ndhB-trnL-CAA_p2	CCAGGAATATCAATGAACCCGA	CTCGCGTGTCTACCATTTCA	45.5	50.0	57.9	58.0	662-713 bp	1.0	68.8
ndhB-trnL-CAA_p3	TCAATTGCTCAGGATACCCT	GTCTCGCGTGTCTACCATTT	45.0	50.0	55.9	58.0	637-688 bp	1.0	68.7
ndhB-trnL-CAA_p4	TCAATTGCTCAGGATACCCT	CTCGCGTGTCTACCATTTCA	45.0	50.0	55.9	58.0	635-686 bp	1.0	68.7
ndhB-trnL-CAA_p5	CCAGGAATATCAATGAACCCGA	TACCATTTCACCACCAAGGC	45.5	50.0	57.9	58.1	652-703 bp	1.0	68.2