Hotspot

ndhD-psaC

SSC · 118409-118549 · 141 bp

Back to Medinilla

Pi 0.0553

MarkerSeek score 89.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 141
variable_sites: 15
pi: 0.0553
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 89.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACCCCCTAATATTGGTAAACCT	AAACAACTCGAAGCATGGGT	40.9	45.0	56.2	58.0	227-252 bp	1.0	55.5
ndhD-psaC_p2	ACCCCCTAATATTGGTAAACCT	GGCATGAAACAACTCGAAGC	40.9	50.0	56.2	58.0	233-258 bp	1.0	55.2
ndhD-psaC_p3	GAACCCCCTAATATTGGTAAACCT	AAACAACTCGAAGCATGGGT	41.7	45.0	58.0	58.0	229-254 bp	1.0	53.8
ndhD-psaC_p4	AGAACCCCCTAATATTGGTAAACC	AAACAACTCGAAGCATGGGT	41.7	45.0	58.0	58.0	230-255 bp	1.0	53.7
ndhD-psaC_p5	GAACCCCCTAATATTGGTAAACCT	GGCATGAAACAACTCGAAGC	41.7	50.0	58.0	58.0	235-260 bp	1.0	53.5